Evaluation of a commercial ELISA test for the detection of a group A rota virus in pig faeces

Authors: Fu ZF
Publication: New Zealand Veterinary Journal, Volume 37, Issue 1, pp 39-40, Mar 1989
Publisher: Taylor and Francis

Animal type: Livestock, Pig, Production animal
Subject Terms: Alimentary system/gastroenterology, Diagnostic procedures, Viral, Disease/defect, Infectious disease
Article class: Correspondence

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Abstract: Rotavirus is still a major cause of gastroenteritis in the young of many species of animals including man. Rotavirus infection is widespread in pig breeding herds and has been associated with milk scours and postweaning diarrhoea. The sensitivity and specificity of a commercial ELISA kit originally developed for use with human faecal samples was compared with electron microscopy (EM) and polyacrylamide gel electrophoresis (PAGE) for the detection of Group A rotavirus in pig faeces. Eighty-nine faecal samples were collected from pigs of various ages from two commercial piggeries and extracted with phosphate buffered saline (PBS) at a ratio of one to four. After homogenization the samples were…

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