Comparison and evaluation of three serological tests for detection of antibodies to infectious bovine rhinotracheitis

Authors: Motha MXJ, Oliver RE, Forbes SD, Cheng-feng Z
Publication: New Zealand Veterinary Journal, Volume 36, Issue 4, pp 204-205, Dec 1988
Publisher: Taylor and Francis

Animal type: Cattle, Livestock, Production animal, Ruminant
Subject Terms: Clinical pathology, Diagnostic procedures, Viral, Syndrome, Respiratory system, Disease/defect, Infectious disease
Article class: Correspondence

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Abstract: Infectious bovine rhinotracheitis (IBR) is caused by bovine herpes virus type 1 (BHV-1). Infection with this virus was shown to be widespread in New Zealand cattle population in a recent serological survey. Freedom from this condition is a criterion for export of cattle from New Zealand to China. Although some countries accept the conventional serum neutralisation test (SNT), where the serum and the virus dilution are incubated for one hour at 37°C (SNT-l), other countries request a 24 hour incubation of serum and virus at 37°C (SNT-2). This study was carried out to compare the two tests, and to determine how these results compared with the indirect enzyme-linked immunosorbent assay (ELISA). SNT-1 and SNT-2 were carried out as described by Rossi and Kiesel and Bitsch respectively. Both tests were…

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