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A rapid tissue extraction method for determining liver copper content by atomic absorption spectroscopy

Authors: Dey RL, Carthew GW
Publication: New Zealand Veterinary Journal, Volume 33, Issue 10, pp 168-170, Oct 1985
Publisher: Taylor and Francis

Animal type: Livestock
Subject Terms: Copper, Trace elements, Diagnostic procedures, Diet/rations/food, Nutrition/metabolism, Poisoning - chemical
Article class: Scientific Article

Abstract: A simple method using tissue extraction of Copper (Cu) in 8M nitric acid for 45 minutes at 100°C, followed by sample dilution and Cu quantitation by flame atomic absorption spectroscopy is described. The detection limit of the method is 16 µmol Cu/kg liver. The mean recovery of added Cu was 100.4%. This method for liver copper analysis correlated well with a method using nitric/perchloric acid digestion and atomic absorption spectroscopy (r² = .986) and is faster, less expensive and less hazardous.

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