A comparison of three serological tests for the diagnosis of B. ovis infection in rams

Authors: Worthington RW, Weddell W, Penrose ME
Publication: New Zealand Veterinary Journal, Volume 32, Issue 4, pp 58-60, Apr 1984
Publisher: Taylor and Francis

Animal type: Livestock, Production animal, Ruminant, Sheep
Subject Terms: Bacterial, Disease/defect, Infectious disease, Clinical pathology, Diagnostic procedures, Notifiable organisms/exotic disease, Reproduction, Zoonosis, Public health
Article class: Scientific Article

---

Abstract: The complement fixation test (CFT), the enzyme labelled immunosorbent assay (ELISA) and the gel diffusion precipitin test (GD) were compared, for the diagnosis of Brucellu ovis infection in rams. The sensitivities of the tests in 109 rams which were shedding B. ovis in their semen were: CFT 96.3%; ELISA 97.2%; GD 91.7%. The specificities of the tests in 141 rams from non-infected flocks were: CFI 99.3%; ELISA 98.6%; GD 100%. Predictive values of the three tests were measured in 285 rams from infected flocks. Thirty-eight percent of these rams were shedding B. ovis in their semen. Predictive values of positive tests were: CFT 75.5%; ELISA 66.7%; GD 72.5%. Predictive values of negative tests were: CFI 97.1%; ELISA 97.6%; GD 93.8%.

---