A modified complement fixation test that is subject to minimal anti-complementary activity by sheep serum

Authors: Jonas W, Stankiewicz M
Publication: New Zealand Veterinary Journal, Volume 28, Issue 8, pp 163-165, Aug 1980
Publisher: Taylor and Francis

Animal type: Livestock, Production animal, Ruminant, Sheep
Subject Terms: Clinical pathology, Diagnostic procedures, Immune system/immunology
Article class: Scientific Article

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Abstract: A warm complement fixation test that will detect antibody in sheep serum in the virtual absence of anti-complementary activity is described. Sheep antibody-antigen complexes were detected by fixation of sheep complement. Sheep serum, heparinized or Mg⁺⁺-EGTA plasma was used as the source of sheep complement. Sheep-antibody-sensitized human erythrocytes were used as the haemolytic indicator cells for sheep complement. As the modified complement-fixation test was performed in the presence of Mg⁺⁺-EGTA, sheep C probably reacts with sheep Ab-Ag complexes by a different mechanism than does guinea-pig complement.

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