The refractometric determination of the total protein concentration in some animal plasmas

Authors: Sutton RH
Publication: New Zealand Veterinary Journal, Volume 24, Issue 7, pp 141-148, Jul 1976
Publisher: Taylor and Francis

Animal type: Cattle, Companion animal, Dog, Horse, Livestock, Production animal, Ruminant
Subject Terms: Clinical pathology, Diagnostic procedures, Nutrition/metabolism, Protein
Article class: Scientific Article

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Abstract: Refractometry has been used for serum or plasma total protein determination since the beginning of this century and while at times the value and accuracy of the technique has been open to question (see Lines and Raine, 1970a,b), many studies have shown close agreement between refractometric determination of serum total protein and estimation by such techniques as the Biuret method and the Kjeldahl nitrogen determination (Lines and Rains, 1970b; Barry et al 1960; Drickman and McKeoa, 1962). Although most of the larger clinical biochemistry laboratories use an automated Biuret melthold for total protein estimation there are many advantages in the use of refractometry for the smaller laboratories where techniques may not be available…

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