The uptake and destruction of Salmonella typhimurium by some body tissues of sheep

Authors: Jonas WE, Broad S, Pulford HD
Publication: New Zealand Veterinary Journal, Volume 21, Issue 1-2, pp 17-25, Jan 1973
Publisher: Taylor and Francis

Animal type: Livestock, Production animal, Ruminant, Sheep
Subject Terms: Bacterial, Alimentary system/gastroenterology, Immune system/immunology, Notifiable organisms/exotic disease, Disease/defect, Infectious disease, Zoonosis, Public health
Article class: Scientific Article

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Abstract: It has been shown that serum obtained from normal sheep or from sheep immunized with Salmonella typhimurium contains a number of proteins that have antibody activity against Salm. typhimurium (Jonas, 1969a, b, c). In addition to the antibody proteins, the involvement of part of the sheep complement (C) system in the reaction of sheep serum with Salm. typhimurium has also been demonstrated (Jonas, 1970). To try to understand the role of the above humoral factors in the way sheep may protect themselves against Salm. typhimurium, attempts were made to lyse this organism with sheep antibody and C (Jonas, unpubl.) but, as with other animals, bacteriolysis was not demonstrated (Osawa and Muschel, 1964). In the process of these experiments, it was observed that normal sheep blood, when incubated in vitro with Salm. typhimurium, would reduce the number of viable organisms. The purpose of this paper is to present the results of experiments involving and sheep blood or spleen cells to show the in vitro requirement of humoral factors in the uptake of Salm. typhimurium by these cells. Some other sheep tissues as well as foetal lamb spleens were also examined for their ability to take up Salm. typhimurium. In addition, guinea-pig antisera raised against sheep tissues has been used in an attempt to determine the possible origin of the cell(s) in the spleen cell preparation involved in the uptake of Salm. typhimurium.

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